org.biojava.bio.seq.impl
Class SimpleSequence

java.lang.Object
  extended by org.biojava.utils.AbstractChangeable
      extended by org.biojava.bio.seq.impl.SimpleSequence
All Implemented Interfaces:
Serializable, Annotatable, FeatureHolder, RealizingFeatureHolder, Sequence, SymbolList, Changeable
Direct Known Subclasses:
PhredSequence, RevCompSequence

public class SimpleSequence
extends AbstractChangeable
implements Sequence, RealizingFeatureHolder, Serializable

A basic implementation of the Sequence interface.

This class now implements all methods in the SymbolList interface by delegating to another SymbolList object. This avoids unnecessary copying, but means that any changes in the underlying SymbolList will be silently reflected in the SimpleSequence. In general, SimpleSequences should only be constructed from SymbolLists which are known to be immutable.

By default, features attached to a SimpleSequence are realized as simple in-memory implementations using SimpleFeatureRealizer.DEFAULT. If you need alternative feature realization behaviour, any FeatureRealizer implementation may be supplied at construction-time.

More functionality and better persistence to biosql is offered by SimpleRichSequence

Author:
Matthew Pocock, Thomas Down, Mark Schreiber
See Also:
Serialized Form

Nested Class Summary
 
Nested classes/interfaces inherited from interface org.biojava.bio.seq.FeatureHolder
FeatureHolder.EmptyFeatureHolder
 
Nested classes/interfaces inherited from interface org.biojava.bio.Annotatable
Annotatable.AnnotationForwarder
 
Nested classes/interfaces inherited from interface org.biojava.bio.seq.FeatureHolder
FeatureHolder.EmptyFeatureHolder
 
Field Summary
 
Fields inherited from interface org.biojava.bio.symbol.SymbolList
EDIT, EMPTY_LIST
 
Fields inherited from interface org.biojava.bio.seq.FeatureHolder
EMPTY_FEATURE_HOLDER, FEATURES, SCHEMA
 
Fields inherited from interface org.biojava.bio.Annotatable
ANNOTATION
 
Fields inherited from interface org.biojava.bio.seq.FeatureHolder
EMPTY_FEATURE_HOLDER, FEATURES, SCHEMA
 
Constructor Summary
SimpleSequence(SymbolList sym, String urn, String name, Annotation annotation)
          Create a SimpleSequence with the symbols and alphabet of sym, and the sequence properties listed.
SimpleSequence(SymbolList sym, String urn, String name, Annotation annotation, FeatureRealizer realizer)
          Create a SimpleSequence using a specified FeatureRealizer.
 
Method Summary
 boolean containsFeature(Feature f)
          Check if the feature is present in this holder.
 int countFeatures()
          Count how many features are contained.
 Feature createFeature(Feature.Template template)
          Create a new Feature, and add it to this FeatureHolder.
 Feature createFeature(FeatureHolder fh, Feature.Template template)
          Deprecated. Please use new 1-arg createFeature instead.
 void edit(Edit edit)
          Apply an edit to the SymbolList as specified by the edit object.
protected  boolean featureHolderAllocated()
           
 Iterator features()
          Iterate over the features in no well defined order.
 FeatureHolder filter(FeatureFilter filter)
          Query this set of features using a supplied FeatureFilter.
 FeatureHolder filter(FeatureFilter ff, boolean recurse)
          Return a new FeatureHolder that contains all of the children of this one that passed the filter fc.
 Alphabet getAlphabet()
          The alphabet that this SymbolList is over.
 Annotation getAnnotation()
          Should return the associated annotation object.
protected  ChangeSupport getChangeSupport(ChangeType ct)
          Called to retrieve the ChangeSupport for this object.
protected  SimpleFeatureHolder getFeatureHolder()
           
 String getName()
          The name of this sequence.
 FeatureFilter getSchema()
          Return a schema-filter for this FeatureHolder.
 String getURN()
          A Uniform Resource Identifier (URI) which identifies the sequence represented by this object.
 Iterator iterator()
          An Iterator over all Symbols in this SymbolList.
 int length()
          The number of symbols in this SymbolList.
 Feature realizeFeature(FeatureHolder parent, Feature.Template template)
          Realize a feature template.
 void removeFeature(Feature f)
          Remove a feature attached to this sequence.
 String seqString()
          Stringify this symbol list.
 void setName(String name)
          Assign a name to this sequence
 void setURN(String urn)
          Provide the URN for this sequence
 SymbolList subList(int start, int end)
          Return a new SymbolList for the symbols start to end inclusive.
 String subStr(int start, int end)
          Return a region of this symbol list as a String.
 Symbol symbolAt(int index)
          Return the symbol at index, counting from 1.
 List toList()
          Returns a List of symbols.
 String toString()
           
 
Methods inherited from class org.biojava.utils.AbstractChangeable
addChangeListener, addChangeListener, generateChangeSupport, hasListeners, hasListeners, isUnchanging, removeChangeListener, removeChangeListener
 
Methods inherited from class java.lang.Object
clone, equals, finalize, getClass, hashCode, notify, notifyAll, wait, wait, wait
 
Methods inherited from interface org.biojava.utils.Changeable
addChangeListener, addChangeListener, isUnchanging, removeChangeListener, removeChangeListener
 
Methods inherited from interface org.biojava.utils.Changeable
addChangeListener, addChangeListener, isUnchanging, removeChangeListener, removeChangeListener
 
Methods inherited from interface org.biojava.utils.Changeable
addChangeListener, addChangeListener, isUnchanging, removeChangeListener, removeChangeListener
 
Methods inherited from interface org.biojava.utils.Changeable
addChangeListener, addChangeListener, isUnchanging, removeChangeListener, removeChangeListener
 

Constructor Detail

SimpleSequence

public SimpleSequence(SymbolList sym,
                      String urn,
                      String name,
                      Annotation annotation)
Create a SimpleSequence with the symbols and alphabet of sym, and the sequence properties listed.

Parameters:
sym - the SymbolList to wrap as a sequence
urn - the URN
name - the name - should be unique if practical
annotation - the annotation object to use or null

SimpleSequence

public SimpleSequence(SymbolList sym,
                      String urn,
                      String name,
                      Annotation annotation,
                      FeatureRealizer realizer)
Create a SimpleSequence using a specified FeatureRealizer.

Parameters:
sym - the SymbolList to wrap as a sequence
urn - the URN
name - the name - should be unique if practical
annotation - the annotation object to use or null
realizer - the FeatureRealizer implemetation to use when adding features
Method Detail

getAlphabet

public Alphabet getAlphabet()
Description copied from interface: SymbolList
The alphabet that this SymbolList is over.

Every symbol within this SymbolList is a member of this alphabet. alphabet.contains(symbol) == true for each symbol that is within this sequence.

Specified by:
getAlphabet in interface SymbolList
Returns:
the alphabet

iterator

public Iterator iterator()
Description copied from interface: SymbolList
An Iterator over all Symbols in this SymbolList.

This is an ordered iterator over the Symbols. It cannot be used to edit the underlying symbols.

Specified by:
iterator in interface SymbolList
Returns:
an iterator

length

public int length()
Description copied from interface: SymbolList
The number of symbols in this SymbolList.

Specified by:
length in interface SymbolList
Returns:
the length

seqString

public String seqString()
Description copied from interface: SymbolList
Stringify this symbol list.

It is expected that this will use the symbol's token to render each symbol. It should be parsable back into a SymbolList using the default token parser for this alphabet.

Specified by:
seqString in interface SymbolList
Returns:
a string representation of the symbol list

subStr

public String subStr(int start,
                     int end)
Description copied from interface: SymbolList
Return a region of this symbol list as a String.

This should use the same rules as seqString.

Specified by:
subStr in interface SymbolList
Parameters:
start - the first symbol to include
end - the last symbol to include
Returns:
the string representation

subList

public SymbolList subList(int start,
                          int end)
Description copied from interface: SymbolList
Return a new SymbolList for the symbols start to end inclusive.

The resulting SymbolList will count from 1 to (end-start + 1) inclusive, and refer to the symbols start to end of the original sequence.

Specified by:
subList in interface SymbolList
Parameters:
start - the first symbol of the new SymbolList
end - the last symbol (inclusive) of the new SymbolList

symbolAt

public Symbol symbolAt(int index)
Description copied from interface: SymbolList
Return the symbol at index, counting from 1.

Specified by:
symbolAt in interface SymbolList
Parameters:
index - the offset into this SymbolList
Returns:
the Symbol at that index

toList

public List toList()
Description copied from interface: SymbolList
Returns a List of symbols.

This is an immutable list of symbols. Do not edit it.

Specified by:
toList in interface SymbolList
Returns:
a List of Symbols

getFeatureHolder

protected SimpleFeatureHolder getFeatureHolder()

featureHolderAllocated

protected boolean featureHolderAllocated()

getURN

public String getURN()
Description copied from interface: Sequence
A Uniform Resource Identifier (URI) which identifies the sequence represented by this object. For sequences in well-known database, this may be a URN, e.g.
 urn:sequence/embl:AL121903
 
It may also be a URL identifying a specific resource, either locally or over the network
 file:///home/thomas/myseq.fa|seq22
 http://www.mysequences.net/chr22.seq
 

Specified by:
getURN in interface Sequence
Returns:
the URI as a String

setURN

public void setURN(String urn)
Provide the URN for this sequence


getName

public String getName()
Description copied from interface: Sequence
The name of this sequence.

The name may contain spaces or odd characters.

Specified by:
getName in interface Sequence
Returns:
the name as a String

setName

public void setName(String name)
Assign a name to this sequence


getAnnotation

public Annotation getAnnotation()
Description copied from interface: Annotatable
Should return the associated annotation object.

Specified by:
getAnnotation in interface Annotatable
Returns:
an Annotation object, never null

countFeatures

public int countFeatures()
Description copied from interface: FeatureHolder
Count how many features are contained.

Specified by:
countFeatures in interface FeatureHolder
Returns:
a positive integer or zero, equal to the number of features contained

features

public Iterator features()
Description copied from interface: FeatureHolder
Iterate over the features in no well defined order.

Specified by:
features in interface FeatureHolder
Returns:
an Iterator

filter

public FeatureHolder filter(FeatureFilter filter)
Description copied from interface: FeatureHolder
Query this set of features using a supplied FeatureFilter.

Specified by:
filter in interface FeatureHolder
Parameters:
filter - the FeatureFilter to apply.
Returns:
all features in this container which match filter.

filter

public FeatureHolder filter(FeatureFilter ff,
                            boolean recurse)
Description copied from interface: FeatureHolder
Return a new FeatureHolder that contains all of the children of this one that passed the filter fc. This method is scheduled for deprecation. Use the 1-arg filter instead.

Specified by:
filter in interface FeatureHolder
Parameters:
ff - the FeatureFilter to apply
recurse - true if all features-of-features should be scanned, and a single flat collection of features returned, or false if just immediate children should be filtered.

containsFeature

public boolean containsFeature(Feature f)
Description copied from interface: FeatureHolder
Check if the feature is present in this holder.

Specified by:
containsFeature in interface FeatureHolder
Parameters:
f - the Feature to check
Returns:
true if f is in this set

realizeFeature

public Feature realizeFeature(FeatureHolder parent,
                              Feature.Template template)
                       throws BioException
Description copied from interface: RealizingFeatureHolder
Realize a feature template. This will be a template which has been passed to the createFeature method of either this FeatureHolder or one of our child Features.

Specified by:
realizeFeature in interface RealizingFeatureHolder
Throws:
BioException

createFeature

public Feature createFeature(Feature.Template template)
                      throws BioException,
                             ChangeVetoException
Description copied from interface: FeatureHolder
Create a new Feature, and add it to this FeatureHolder. This method will generally only work on Sequences, and on some Features which have been attached to Sequences.

Specified by:
createFeature in interface FeatureHolder
Throws:
BioException - if something went wrong during creating the feature
ChangeVetoException - if this FeatureHolder does not support creation of new features, or if the change was vetoed

getSchema

public FeatureFilter getSchema()
Description copied from interface: FeatureHolder
Return a schema-filter for this FeatureHolder. This is a filter which all Features immediately contained by this FeatureHolder will match. It need not directly match their child features, but it can (and should!) provide information about them using FeatureFilter.OnlyChildren filters. In cases where there is no feature hierarchy, this can be indicated by including FeatureFilter.leaf in the schema filter.

For the truly non-informative case, it is possible to return FeatureFilter.all. However, it is almost always possible to provide slightly more information that this. For example, Sequence objects should, at a minimum, return FeatureFilter.top_level. Feature objects should, as a minimum, return FeatureFilter.ByParent(new FeatureFilter.ByFeature(this)).

Specified by:
getSchema in interface FeatureHolder
Returns:
the schema filter

createFeature

public Feature createFeature(FeatureHolder fh,
                             Feature.Template template)
                      throws BioException,
                             ChangeVetoException
Deprecated. Please use new 1-arg createFeature instead.

Create a new feature in any FeatureHolder associated with this sequence.

Throws:
BioException
ChangeVetoException

removeFeature

public void removeFeature(Feature f)
                   throws ChangeVetoException,
                          BioException
Remove a feature attached to this sequence.

Specified by:
removeFeature in interface FeatureHolder
Throws:
ChangeVetoException - if this FeatureHolder does not support feature removal or if the change was vetoed
BioException - if there was an error removing the feature

edit

public void edit(Edit edit)
          throws ChangeVetoException
Description copied from interface: SymbolList
Apply an edit to the SymbolList as specified by the edit object.

Description

All edits can be broken down into a series of operations that change contiguous blocks of the sequence. This represent a one of those operations.

When applied, this Edit will replace 'length' number of symbols starting a position 'pos' by the SymbolList 'replacement'. This allow to do insertions (length=0), deletions (replacement=SymbolList.EMPTY_LIST) and replacements (length>=1 and replacement.length()>=1).

The pos and pos+length should always be valid positions on the SymbolList to:

Examples

 SymbolList seq = DNATools.createDNA("atcaaaaacgctagc");
 System.out.println(seq.seqString());

 // delete 5 bases from position 4
 Edit ed = new Edit(4, 5, SymbolList.EMPTY_LIST);
 seq.edit(ed);
 System.out.println(seq.seqString());

 // delete one base from the start
 ed = new Edit(1, 1, SymbolList.EMPTY_LIST);
 seq.edit(ed);

 // delete one base from the end
 ed = new Edit(seq.length(), 1, SymbolList.EMPTY_LIST);
 seq.edit(ed);
 System.out.println(seq.seqString());

 // overwrite 2 bases from position 3 with "tt"
 ed = new Edit(3, 2, DNATools.createDNA("tt"));
 seq.edit(ed);
 System.out.println(seq.seqString());

 // add 6 bases to the start
 ed = new Edit(1, 0, DNATools.createDNA("aattgg");
 seq.edit(ed);
 System.out.println(seq.seqString());

 // add 4 bases to the end
 ed = new Edit(seq.length() + 1, 0, DNATools.createDNA("tttt"));
 seq.edit(ed);
 System.out.println(seq.seqString());

 // full edit
 ed = new Edit(3, 2, DNATools.createDNA("aatagaa");
 seq.edit(ed);
 System.out.println(seq.seqString());
 

Specified by:
edit in interface SymbolList
Parameters:
edit - the Edit to perform
Throws:
ChangeVetoException - if either the SymboList does not support the edit, or if the change was vetoed

toString

public String toString()
Overrides:
toString in class Object

getChangeSupport

protected ChangeSupport getChangeSupport(ChangeType ct)
Description copied from class: AbstractChangeable
Called to retrieve the ChangeSupport for this object.

Your implementation of this method should have the following structure:

 ChangeSupport cs = super.getChangeSupport(ct);

 if(someForwarder == null && ct.isMatching(SomeInterface.SomeChangeType)) {
   someForwarder = new ChangeForwarder(...

   this.stateVariable.addChangeListener(someForwarder, VariableInterface.AChange);
 }

 return cs;
 
It is usual for the forwarding listeners (someForwarder in this example) to be transient and lazily instantiated. Be sure to register & unregister the forwarder in the code that does the ChangeEvent handling in setter methods.

Overrides:
getChangeSupport in class AbstractChangeable